Cultural Methods of Isolation of Tubercle Bacilli

Selective media in the isolation of tubercle bacilli from tissues.

Direct culture on Lowenstein-Jensen slopes and on three media made selective for tubercle bacilli by the addition of four antibacterial agents was compared with guinea-pig inoculation on 490 tissue specimens. Tubercle bacilli were obtained from 15 specimens by culture and 14 by guinea-pig inoculation; only one specimen was positive by guinea-pig and not by culture. The most efficient culture me...

Streptomycin-dependent Tubercle Bacilli: a Simple Method for Isolation.

This report describes a simple method for the isolation of streptomycin-dependent tubercle bacilli from virulent cultures that have not previously been exposed to streptomycin. The in vivo isolation of streptomycin-dependent Mycobacterium tuberculosis has been reported by Spendlove et al. (1948) and Lenert and Hobby (1949). The in vitro procedure described here is applicable to the quantitative...

DIFFERENTIATION OF LEPROSY AND TUBERCLE BACILLI. 219 On the Differentiation of Leprosy and Tubercle Bacilli

On the nucleoproteins of avian tubercle bacilli.

The nucleic acids of tubercle bacilli have formed the subject of several studies, among which may be mentioned those by Ruppel (l), Levene (2), and Johnson and his associates (3-5). The work of Mensel and Heidelberger (6) on the fractionation of the proteins of the tubercle bacillus revealed the presence of several protein fractions, rich in phosphorus and in purines, which appeared to be nucle...

The Effect of Spermine on Tubercle Bacilli

A crystalline substance capable of suppressing the growth of a variety of mycobacteria in vitro has been isolated from extracts of tissue in acidified dilute ethanol. This inhibitory material was found to be equally active against virulent, attenuated, and avirulent variants of human and bovine tubercle bacilli, but had little or no effect on saprophytic mycobacteria and on several non-acid-fas...